Part:BBa_K4025006

ChiSPY (Chitin-Sensing Pathway Biosensor Device)

This chitin-sensing device is made up of four proteins: ChiP, CBP, ChiS, and sfGFP regulated by the Pchb promoter. The ChiSPY device uses proteins involved in the Vibrio cholerae chitin-sensing one-component system to express GFP when induced by the presence of chitin.

Growth curves were constructed for the L1 transcription units (ChiP, CBP, ChiS, and sfGFP) that make up the ChiSPY device. See: https://2021.igem.org/Team:Lubbock_TTU/Contribution

As can be seen from the growth curves none of the L1 transcriptional units overly burdened cell growth– all transformed cells survived. It should be noted, however, that the OD600 readings of all cultures were not standardized prior to inoculation, seen by the varying y-intercepts of all growth curves. Because of this, this data should not be used as quantitative measurements of growth kinetics, but rather a qualitative demonstration of relative growth.